Isolation and Purification of Casein from Iraqi Camels′ Milk (Camelus dromedaries) and Evaluation of Its Activity Against Some Types of Cancer Cells in vitro

Qais Al-Ani, Mohammed

	Isolation and Purification of Casein from Iraqi Camels′ Milk (Camelus dromedaries) and Evaluation of Its Activity Against Some Types of Cancer Cells in vitro
Iraqi Journal of Cancer and Medical Genetics
Article 1, Volume 6, Issue 1, 2013, Pages 93-100
Author
Mohammed Qais Al-Ani
Abstract
This study aimed to isolate and purify the casein from Iraqi camels′ milk and evaluating its activity against some types of cancer cells in vitro . The study included the isolation of casein in acidic medium (pH=4.6) and its purification in DEAE-Cellulose ion exchanger and Sephadex G-200 Gel filtration .To verify the purity of isolated casein, electrophoresis has been achieved on PAGE. To specify the immunological behavior for the partially purified casein , immunoblotting has been achieved by Western Blot . To recognize the cytotoxicity of casein , High Screening Content (HSC) against Human Lung Adenocarcinoma A549 has been achieved . To evaluate the inhibiting ability against four types of cancer cells (A549 , MCF7 , PC3 , HePG2) . The availability of cell has been determined by MTT test.The results showed that casein had three peaks on DEAE-Cellulose and Sephadex G-200 for each column . By the use of electrophoresis on PAGE , a band similar to standard casein appeared . Also , casein showed immunological behavior depending upon the concentration in western blot test. The results showed that casein had inhibiting ability against cancer cells the proportion was reversal between casein and cancer cells availability . Casein had different inhibition activity against cancer cells content. We conclude , partially purified casein from Iraqi camels′ milk had inhibiting activity against certain types of cancer cell in vitro

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