- All Journals
- Al-Qadisiyah University
- Basrah University
- Kirkuk University
- Mosul University
- The Iraqi Borad for Medical Specialization
- University of Anbar
- University of Technology
- Al Mustafa University
- Al-Zahraa University for Women
- Alnoor University
- Kunooz University
- Misan University
- Ninevah University
- Tikrit University
- University of Al-Hamdaniya
- University of Kerbala
Isolation and Identification The Actinomycetes From Soil and Its Effect on Human Fungi | ||
| journal of kerbala university | ||
| Article 1, Volume 1, Issue 0, 2005, Pages 106-113 PDF (0 K) | ||
| Abstract | ||
| One hundred and nine isolates of actinomycetes were obtained from 104 soil sample, collected over three months during 10-01-2010 from different regions of the province of Nineveh,Duhok,Irbil,Kirkuk,Tikrit, Baghdad, Najaf and Karbala. Results of diagnosis by Slide Culture Technique and by biochemical and enzymatic test have showed the existence of the following isolates and races (69 isolates belong to the genus of Streptomyces, 27 isolates belong to the genus of Streptoverticillum, 7 isolates belong to the genus of Nocardia and one belong to the genus of Rhodococcus, and 5 isolates have not been diagnosed because of the difficulty of growth on the diagnostic and biochemical media). The productive isolate which have inhibited the largest number of fungi have been diagnosed to the level of species was S.purpureus. Six isolates of which have showed their ability to produce antifungi used in the test. These isolates are: MUAc.1, MUAc.2, MUAc.3, MUAc.4, MUAc.5 and MUAc.6 which belong to the genus of Streptomyces. The isolate MUAc.3 had the capacity to inhibit the greatest number of tested fungi where it effect of the growth of 14 human pathogenic fungi , that's to say 27 fungi out of 28 ,The isolate MUAc.3 affected all tested fungi except Aspergillus flavus fungi. Following by isolates MUAc.1 , MUAc.2 , MUAc.5 which inhibited the growth of 9 human pathogenic fungi , isolate MUAc..4 inhibited the growth of 7 human pathogenic fungi, and finally isolate MUAc.6 inhibited the growth of 6 human pathogenic . The highest inhabitation to the extracted antibiotic from isolate MUAc.3 was on the fungi of A.amstelodami where it reached 36mm for Nystatin which was used for the comparison. the lowest inhabitation for the antibiotic extracted from isolate MUAc.3was on A.candidus. (21 mm) and with Nystatin 24 mm . The effectiveness of the antibiotic extracted from isolate MUAc.3 was (10455) IU/mg, it was measured by using the given effectiveness of Nystatin amounting 8500 IU/mg by using constitutional pharmaceutical method. This was done by using A.amstelodami fungi. Isolate MUAc.3 diagnosed to the level of species S.purpureus. | ||
|
Statistics Article View: 176 PDF Download: 105 |
||