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The effects of Trehalose on the semen cryopreservation of Awasse ram | ||
| Al-Anbar Journal of Veterinary Sciences | ||
| Article 1, Volume 6, Issue 1, 2013, Pages 14-20 PDF (239.33 K) | ||
| Authors | ||
| H. K. J. Al-Saab; A. A. Hubi | ||
| Abstract | ||
| The purpose of this study was to determine the influence of Trehalose added to the freezing media on the semen cytological parameters post-thawing (motility, viability, fertility). The experiments were done on 32 ejaculates collected by artificial vagina from four Awassi rams aged 2.5-3 years as 2 ejaculate/ ram/ week. After collecting, the samples were pooling and diluted (1:20) in medium based on Tris in which Trehalose were added (100 mM/ml) or in medium without Trehalose (control). The diluted semen was cooled at 4Cº, placed in fine 0.25 ml French straws and then stored in liquid nitrogen, Thawing of the straws were done to evaluated the semen and to know the viability of sperm with 1-6 hours after thawing. invitro fertilization (IVF) used to evaluated the cryopreservation sperms. The results indicated that, there were a significant effect (p<0.05) for Trehalose (100 mM/ml) on the motility of sperms after thawing, which were 33.13±0.91 and 10.63±1.48% for treatment and control group respectively. There was no effect for the Treatments (Trehalose 100 mM/ml) on the invitro fertilization of sperms after thawing, which were 15±2.00 and 16.31±2.99% for both groups respectively. | ||
| Keywords | ||
| trehalose; cryopreservation; Rams | ||
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