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The extraction of Low Density Lipoprotein (LDL) and used in the diluent for the cryopreservation of Awassi ram semen | ||
| Al-Anbar Journal of Veterinary Sciences | ||
| Article 1, Volume 6, Issue 1, 2013, Pages 35-43 PDF (267.76 K) | ||
| Authors | ||
| H. K. J. Al-Saab; A. A. Hubi | ||
| Abstract | ||
| This experiment was conducted in order to improve sperm quality. For limiting cryopreservation harmfull effect, Low Density Lipoprotein (LDL) as a good replacement for normal egg yolk was used. Semen samples was collected from four Awassi rams aged 2.5-3 years collected by the artificial vagina as 2 ejaculate/ram/week. Ejaculated ram sperm was pooling and diluted (1:20) with two deferent diluent (first: tris with 8% LDL and second: tris with 20% egg yolk), the cryopreservation of ram semen was done by the liquid nitrogen in the artificial insemination center, Thawing of the straws were done to evaluated the semen and to recorded the viability of sperm from 1- 6 hours after thawing after one, two and three months of cryopreservation. Invitro- fertilization (IVF) was used to evaluated the sperm cryopreservation. The results indicated: The viability of sperms significantly higher (p<0.05) (51.88 ± 3.26%) with semen frozen in 8% LDL extender compared to 20% egg yolk (33.13 ± 0.91) after the freeze-thaw process. The semen diluted with the extender with 8% LDL concentration Significantly (p<0.05) enhanced invitro fertilization after thawing | ||
| Keywords | ||
| low density lipoprotein; cryopreservation; Rams | ||
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