Mohammed Zedan, S., Haza, D., rasheed, A. (2013). Callus Cultures derived from electrotreated cell suspension of Dianthus caryophyllus L. embedded in agar drops express their totipotency. , 7(2), 32-38.
Sahla Mohammed Zedan; Djamela Haza; a rasheed. "Callus Cultures derived from electrotreated cell suspension of Dianthus caryophyllus L. embedded in agar drops express their totipotency". , 7, 2, 2013, 32-38.
Mohammed Zedan, S., Haza, D., rasheed, A. (2013). 'Callus Cultures derived from electrotreated cell suspension of Dianthus caryophyllus L. embedded in agar drops express their totipotency', , 7(2), pp. 32-38.
Mohammed Zedan, S., Haza, D., rasheed, A. Callus Cultures derived from electrotreated cell suspension of Dianthus caryophyllus L. embedded in agar drops express their totipotency. , 2013; 7(2): 32-38.

Callus Cultures derived from electrotreated cell suspension of Dianthus caryophyllus L. embedded in agar drops express their totipotency

Jornal of Biotechnology Research Center
Article 1, Volume 7, Issue 2, 2013, Pages 32-38
Authors
Sahla Mohammed Zedan; Djamela Haza; a rasheed
Abstract
Callus cultures were produced from leaf, excised from axenic carnation, Dianthus caryophyllus L. seedling, on agar-solidified MS medium supplemented with 0.5 mgl-1 of 2,4-D and 0.1 mgl-1 of BA. Cell suspension was initiated in liquid MS medium containing the above growth regulators. This suspension reported the density of 143 × 103 cell /ml and its viability was ranged between 41-90%. Samples of cell suspension represents seven different densities were exposed to three electrotreatment 300v/50 msec, 500v/100msec. and 700v/150 msec. These electrotreated samples were cultured by embedding in to agar drops in solid- liquid cultures. Callus derived from cell suspension expressed their totipotency forming carnation plants.
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