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The typing and the mitogenic potentials of Lens cinarus and Phaselius aureus seed lectins | ||
| Journal of University of Babylon | ||
| Article 1, Volume 20, Issue 2, 2012, Pages 525-533 | ||
| Authors | ||
| Sura I. A. Jabuk; Qasim N.O.Thewaini; Ibrahim M-S.A-W. Shnawa | ||
| Abstract | ||
| Legium Lectins are widely used as a model system for protein- protein, protein-carbohydrate interactions. So two legium seeds were elected, as Lens cinarius and Phaselius aureus Roux for detection of their lectins .Such lectins were extracted, separated and partially characterized as LCL and PAL. respectively. Two prokaryotic cellular systems Klebsiella pneumoniae and Escherichia coli and two eukaryotic cellular systems the human erythrocytes as well as the avian T-lymphocyte, were elected. Cell typability were studied on K.pneumoniae, E.coli and human erythrocytes , while T-cell mitogenicity were checked by lectin skin test in avian wing system. Two typing patterns were observed in both K. pneumoniae and E.coli. togther with LCL untypable isolates of K. pneumoniae and E.coli . Pantypability was identified in human erythrocyte in both of LCL and PAL. Positive mitogenic responses were noted in the avian wing test. Thus, both LCL and PAL have typing and mitogenic potentials. Which could be due protein- protein or protein-carbohydrate interactions. الخلاصة | ||
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