Protective Effect of Vitamin A against Oxidative Stress Caused by Methotrexate

I. Sail, Asmaa; A. Al-Mahdawi, Ferial; Q. Al-Lami, Makarim; M. Al-Jalabi, Salah

	Protective Effect of Vitamin A against Oxidative Stress Caused by Methotrexate
Iraqi Journal of Science
Article 1, Volume 54, Issue 3, 2013, Pages 585-589
Authors
Asmaa I. Sail; Ferial A. Al-Mahdawi; Makarim Q. Al-Lami; Salah M. Al-Jalabi
Abstract
Methotrexate (MTX), a folate antagonist agent, is mainly used in treatment of malignant tumors and autoimmune diseases. The present study was undertaken to determine whether antioxidant vitamin (vitamin A) could ameliorate methotrexate-induced oxidative stress in male rabbits. Twenty male rabbits were randomly assigned into four groups. Group 1: control group, Group 2: MTX-treated group (received 20 mg/kg MTX intraperitoneally), Group 3: Vit.A treated group received 5000 IU Vit.A orally) and Group 4: MTX+Vit.A treated group received MTX 20 mg/kg plus 5000 IU vit.A). After 4 weeks of treatment, blood samples were collected by cardiac puncture to determine the serum malondialdehyde (MDA), as a good indicator for lipid peroxidation and oxidative stress. The results showed that there was a significant increase (p<0.05) in serum MDA level in MTX-treated group compared with control group. Also, the results revealed that there was a significant decrease (p<0.05) in serum MDA level in MTX+Vit.A treated group in comparison with MTX-treated group. The present study suggests that the administration of vit.A with MTX is associated with reduction in oxidative stress, and therapeutic role in reduced toxicity of MTX.
Keywords
methorexate; vitamin A; Malondialdehyde; Oxidative stress

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