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The anti- microbial activity of the aquatic, alcoholic and volatile oil extracts of rosa damascena l. Petals | ||
| Kirkuk Journal of Science | ||
| Article 1, Volume 8, Issue 1, 2013, Pages 25-43 PDF (0 K) | ||
| DOI: 10.32894/kujss.2013.77600 | ||
| Author | ||
| Bushra Ali Kadhim | ||
| Abstract | ||
| The Petals of Rosa damascena L. had used in this research to prepare three types of extracts which were aquatic, alcoholic and volatile oil . A set of chemical tests were made on these prepared extracts, as well as, using the Fourier Transform Infrared spectrophotometer to detect the active positions in these extracts. The results showed the foundation of some active compounds (Tannines, Alkaloides, Saponins, Flavonoides, Terpens, Steroids and Volatile Oil) in prepared extracts of Rosa damascena L., as well as, the anti-microbial activity of these extracts was tested on eleven types of microorganisms (contain seven types of bacteria and four types of fungi) which were isolated from burns and wound infections. The resulls of stastical analysis by using L.S.D. test at probability level 0.05 had proved that the concentrations 100, 250 mg/ml of aquatic extract had considered as the MIC and MBC for Proteus mirabilis, Escherichia coli and Candida albicans respectively, while the two concentrations 50, 250 mg/ml of aquatic extract had considered as the MIC and MBC for Staphylococcus aureus , Burkhorderia mallei and Candida tropicalis respectively, and about the concern of Proteus vulgaris and Epidermophyton floccosum , the concentration 125 mg/ml had inhibited their growth and the concentration 250 mg/ml had killed them . Also the results affirmed there was no affect of aquatic extracts on Pseudomonas aeruginosa and Trichophyton mentagrophytes in all prepared concentrations. The results showed that the two concentrations 16, 32 mg/ml of alcoholic extract were inhibited and killed for Proteus mirabilis and Pseudomonas flouresence respectively, while the two concentrations 4, 32 mg/ml of the same extract were inhibited and killed for Burkhorderia mallei, Escherichia coli, Candida albicans, as well as, Candida tropicalis respectively. The results of this research also indicated that the growth of Epidermophyton floccosum , Trichophyton mentagrophytes , Staphylococcus aureus and Proteus vulgaris was inhibited and killed by the alcoholic extract at the concentrations (8, 16), (8, 32), (4, 16) and (8, 32) mg/ml respectively. As well as, the results reffered to the inhibitory activity of volatile oily extract on the growth of all microorganisms under study at the concentrations between (1:2) and (1:3), while the killed concentrations between (1:3) and (1:4). | ||
| Keywords | ||
| The Petals of Rosa damascene aquatic; alcoholic and volatile oil; chemical test | ||
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