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Gas Chromatography-Mass Spectrometry (GC-MS) Analysis of Active Compounds Produced from Rosemary (Rosmarinus Officinalis L.) Callus and Leaf Extracts | ||
| Al-Mustansiriyah Journal of Science | ||
| Article 1, Volume 21, Issue 4, 2010, Pages 111-118 | ||
| Author | ||
| Salah K. Mahmoud | ||
| Abstract | ||
| The yield of rosemary (Rosmarinus officinalis L.) from active compounds was investigated. Yield of callus tissue was compared with the intact plant production. Callus was induced in leaf explants and maintained on Murashige and Skoog medium (MS) supplemented with 2,4-D and BA. Maximum callus fresh weight was obtained in the combination of 2 mg/l 2,4-D and 0.5 mg/l BA under 16/8 hrs photoperiod, which reached 1780 mg. The GC-MS analysis of leaf ethanolic extracts revealed the most abundant components; isocarnosol (5.85%), camphor (3.88%), ferruginol (4.53%), cineole (2.66%), verbenone (2.50%) and borneol (1.50%). The chemical analysis of callus ethanolic extracts showed the most compounds but at higher percentages than in leaf extracts. Quinoline alkaloids, were found for the first time in callus have been of great therapeutic value in pharmaceutical industries. | ||
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