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The Antibodies Titer of Anti-Serratia marcescens Protease by Passive Hemagglutination | ||
| Journal of University of Babylon | ||
| Article 1, Volume 20, Issue 4, 2012, Pages 1159-1166 | ||
| Authors | ||
| Raghad H. Al-azzawi; Layla Fouad Ali; ; a Nabhan | ||
| Abstract | ||
| Serratia marcescens isolated from a patient with urinary tract infection taken from the Central Health Laboratory. The sensitivity test showed the resistant to the Tetracyclin (30 µg), Amoxicillin (25 µg), Gentamycin(30 µg), ampicillin (10µg), and Kanamycin (30µg), but it was sensitive to Ciprofloxacin (5 µg) only. The extracellular protease was extracted in LB broth and purified in simple two step included precipitation with (30-55%) saturation of ammonium sulfate, dialysis and ion exchange chromatography by DEAE – Cellulose. Only one peak contained the enzymatic activity, and purification fold was 22.6 with 37.92% recovery. The result of passive haemagglutination indicated that the optimum concentration of Protease was (7.5) µg/ ml which gave positive reaction with a highest dilution of serum in(1/ 320). | ||
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