Initiation  of  Biofilm  Formation  by  Pseudomonas aeruginosa  Serotype  and  Pseudomonas oryzihabitans Correlates with Emergence of Hyperpiliated and Highly Adherent in Swimming ,Swarming ,and Twitching Motilities

Khalid Mohammed, Manal

	Initiation of Biofilm Formation by Pseudomonas aeruginosa Serotype and Pseudomonas oryzihabitans Correlates with Emergence of Hyperpiliated and Highly Adherent in Swimming ,Swarming ,and Twitching Motilities
Al-Mustansiriyah Journal of Science
Article 1, Volume 22, Issue 7, 2011, Pages 1-14
Author
Manal Khalid Mohammed
Abstract
The (44) isolates were obtained from clinical specimens in Baghdad hospital and the duration of study during the two months ( April and May) , the (14) isolates of Pseudomonas aeruginosa serotype and ( 3) isolates of Pseudomonas oryzihabitans were after identified biochemically test, Epi20 analysis and serotyping analysis . The clinical isolates of P.aeruginosa serotype and P. oryzihabitans are abiquitous environmental bacterium capable of forming surfaces as a survival strategy it exhibit , a large variety of competition / virulence factors , such as three types of motilities :- flagellum mediated swimming , flagellum mediated swarming , and type IV pilus mediated twitching . Resultes were showed that these two types of species produced abundant type IV fimbriae, displayed swimming , swarming ,and twitching motilities and were impaired in chemotaxis . Some strains of P.aeruginosa serotype (P11,P12,P16) and P. oryzihabitans displayed defective in this motilities therefore this strains were poorly adherent homogeneously dispersedin liquid cultures. While another strains are autoaggregated in liquid cultures and rapidly initiated the formation of strongly adherent biofilms . Further analysis of the two species was displayed a differences in chemo tactic response in the same serotype the fact that all three types of motilities were affected in addition to the absence of clear chemo tactic rings on swim plates , suggested a defect in chemotaxis .The chemo tactic results to the trypton between (1-1.5) are not significant while the results between (2-5) are significant .Then the study showed that all the strains exhibit sensitivity to oxidative stress. In the infected host bacterial biofilms are resistant to host defense and antibiotic treatment therefore in this report is study the antibiotic susceptibility of the two species of Pseudomonas and the results of this study are 100% resistant to antibiotics (amikacin, ciprofloxacin, and meropenem) , 88% for cefoperazone , 59% for ceftazidim, 47% for tobramycin ,and 23% ,35% for colistin and levofloxacin respectively.

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