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A Study of Antibacterial Activity of Fatty Acids Extracted from Pseudomonas sp. LP1 by using Chromatography technique | ||
| Journal of University of Anbar for Pure Science | ||
| Article 12, Volume 6, Issue 3, 2012, Pages 40-45 | ||
| Document Type: Research Paper | ||
| DOI: 10.37652/juaps.2012.78441 | ||
| Author | ||
| Kutayba F. Dawood* | ||
| University of Anbar -College of Education for pure science. | ||
| Abstract | ||
| A bacterium that was capable of metabolizing C1- compounds was isolated and identified in Moscow State Academy of Fine Chemical Technology (Russia), as Pseudomonas sp. LP1 based on detailed taxonomic studies. This organism is a new strain of obligate methylotrophic bacteria, which was cultured on the methanol-containing medium (1%) as its sole carbon source. This study included culture of Pseudomonas sp. LP1 in M9 modified medium. The lipids were extracted from biomass of bacteria by organic solvents where a mixture of fatty acid methyl esters (FAMEs) was obtained. Antibacterial activity of total fatty acids was tested against some standard strains of both gram positive and gram negative bacteria by using wells in agar plates. Fatty acids mixture were found to have antibacterial effects. Thin-layer chromatography was used to tentatively identified the mixture components of fatty acids. A gas-liquid chromatographic system referred that Palmitic (16:0), Palmitoleic (16:1) and Oleic (18:1) acids were present. | ||
| Keywords | ||
| Pseudomonas sp; Methylotrophic bacteria; Fatty acid methyl esters | ||
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