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Partial Purification of phenylalanine hydroxylase from urine of patients with chronic renal failure | ||
| karbala journal of pharmaceutical sciences | ||
| Article 1, Volume 4, Issue 5, 2013, Pages 183-194 PDF (0 K) | ||
| Authors | ||
| ; F. G; A. H; ; ; A. K | ||
| Abstract | ||
| This study include purification of Phenylalanni hydroxylase ( PAH) from patients urine with chronic renal failure. The purification steps include,protein precipitation with ammonium sulphate solution. And ion exchange chromatography by using (DEAE – Cellulose A – 50) as anionic exchanger . Then two isoenzymes (I,II) were seperated through gel filtration by using (sephadex G-200) with purification folds 20.5 & 23.7 respectivily . the research also include the kinetics studies of the two partially purified isoezymes (I&II) . The Km values were 4 mM .The two isoenzymes shown an optimum pH at 7.2, and optimum temperature at 25 Cº. | ||
| Keywords | ||
| Phenylalanine hydroxylase; Purification | ||
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