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Isolation and Identification of Azospirillum spp.from some plants grow in gypsi ferous soil and evaluation of its effecincy for Nitrogen fixation and IAA production | ||
| Journal of Tikrit University For Agriculture Sciences | ||
| Article 1, Volume 13, Issue 2, 2013, Pages 310-320 | ||
| Abstract | ||
| A field surveying of three regions including Non-Rhizosphere, Rhizosphere and Rhizoplan was carried out in Agriculture college fields/ Tikrit university. 162 soil and roots samples were collected from six host plants including wheat Triticum aestivum , barley Hordeum vulgare , sorghum sp., soybean Glycine max, alfalfa Medicago sativa , and broad bean Vicia faba during three months from the plant age for the study of population level of Azospirillum associated with these plants grown on gypsiferous soil. The results showed isolation of 155 isolates belong to four species A.brasilense, A. halopraeferens , A. lipoferum and A. irakense which diagnosed according to microscopic and biochemical properties. the highest frequent species was A.brasilense (89%) followed by A. halopraeferens (22.5%) then , A. lipoferum (19.3%) and A. irakense (0.6%). In this study, isolation of A. halopraeferens was recorded for the first time in Iraqi soil in addition, isolation of A. irakense was recorded for the first time in Salah-Aldin government.The results showed the maximum nitrogen fixation and IAA production were recorded by A.brasilense followed by A. lipoferum then , A. halopraeferens and A. irakense . The highest nitrogen fixation and IAA production were recorded by the isolate AB83T of A.brasilense (14.7 mg/L and 10.6µg/ml) while in A. lipoferum the isolate Al28T gives (13.2 mg/L and 9. 6µg/ml , then the isolate Ah16T of A. halopraeferens (13 mg/L and 9.8 µg/ml ) and the isolate of A. irakense (11 mg/L and 8.3 µg/ml), respectively.The results also showed the highest percentage of Azospirillium species frequent was 74% in rhizoplan followed by 65.1% in rhizosphere, while the lower percentage was 51% in non-rhizosphere. According to the hosts, the highest percentage of Azospirillium species frequent was 88.1,80.7,71, 54, 50.3 and 36.2% in wheat, barley, sorghum, alfalfa , broad bean and soybean, respectively. | ||
| Keywords | ||
| identification; plants; soil; Production | ||
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