The objectives of this study were to investigate the effect of oxidative stress induced by H2O2 (0.5%) supplemented with drinking water on the semen quality fertility and hatchability treats and antioxidant status of (Arbor Acres ) broiler breeder males . In addition of study effect Vitamin A . Oxidative stress were determined by measuring the level of glutathione (GSH) and malondialdehyde (MDA) in the testis tissues and seminal plasma .Sixty (Arbor Acres) broiler breeder males at 26 weeks of age were used in this study .The birds were randomly assigned in to three treatments groups (2 replicates, each with 10 birds), as follows :
T (1) The birds were given standard diet +normal water .T (2) The birds were given the same ration as treatment (1) + H2O2 (0.5%) in drinking water . T (3) The birds were given a standard ration + 15 mg vitamin A/Kg feed + normal water . in MDA and a decrease in GSH levels of both the testis and the seminal plasma . Significant increases (P≤0,05) in dead and abnormal sperms percentages. And of the embryonic mortality rate as compared with other treatments, were noted. An improvement of the antioxidant status of both the testis tissue and seminal plasma were evident in birds receiving vitamin A , as represented through an increase of GSH and decrease in MDA levels , this effected Positively the male reproductive performance through significantly increasing (P≤0,05) the mass activity , individual motility , sperm concentration , fertility percentage , hatching rate from total eggs number and hatching rate from fertile eggs compared with H2O2 treatment The treatment period length for H2O2 supplementation had no significant effect on ejaculation volume , sperm concentration.There were a significant effects for period length of vitamin A treatment in all semen's characteristics .
It was concluded from the present study that supplementing (H2O2) had resulted in an increase in the oxidative stress of broiler breeder males and the harmful negative effect was evident during the period of the treatment The treatments with vitamin A lead to improve of the antioxidant status and most semen's characteristics |