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Spectrophotometric Determination of Teicoplanin via Coupling with Diazotized p-Nitroaniline | ||
| Rafidain Journal of Science | ||
| Article 5, Volume 24, Issue 9, 2013, Pages 42-51 PDF (0 K) | ||
| DOI: 10.33899/rjs.2013.78758 | ||
| Authors | ||
| Nabil H. Othman; Nabeel S. Othman | ||
| Abstract | ||
| A new, simple and sensitive spectrophotometric method was described for the determination of teicoplanin as a pure material and in pharmaceutical formulation. The method is based on the coupling of teicoplanin with diazotized p-nitroaniline in alkaline medium. The dye shows an absorption maximum at 490 nm and obeys Beer's law over a range of 2 to 80 µg.ml.-1 of teicoplanin. The molar absorptivity of the coloured azo dye is 2.8 x 104l. mol-1cm-1. The relative standard deviation does not exceed 2.7 % (n=5). The azo dye formed is stable for 60 minutes. The method was applied successfully to the assay of teicoplanin in pharmaceutical preparation (injection). | ||
| Keywords | ||
| Teicoplanin; Determination; spectrophotometry; diazo; Coupling | ||
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