Outbreak of Multidrug-Resistant Pseudomonas aeruginosa Isolates from Burn Infections and Detection of Some  Virulence Factors

Mun,; R. Ali, Im

	Outbreak of Multidrug-Resistant Pseudomonas aeruginosa Isolates from Burn Infections and Detection of Some Virulence Factors
Al-Mustansiriyah Journal of Science
Article 1, Volume 20, Issue 4, 2009, Pages 1-10
Authors
; im R. Ali
Abstract
In this study, during November 2007 till April 2008, 320 swaps from different wound infections were collected from different Baghdad Hospitals, (only one smear per patient was taken). Numbers of swaps showed bacterial growth were 276. From which (215) isolates belong to gram negative bacteria, 61isolates referred to gram positive bacteria, while 44 swaps didn’t showed any bacterial growth. Primary identification depending on morphological and biochemical tests were performed followed by using api20E to confirm isolation. The results showed that from the total 215 gram negative isolate only 48 swaps were colonized with Pseudomonas aeruginosa. The present study revealed that there were high rate of resistance against 16 different antibiotics were used, 100% of P. aeruginosa showed resistance to Penicillin, Pipracillin, Amoxicillin, Cefalotin Cefixime, Ceftriaxone, Ceftazidiume and Cefepime ,the Polymixin was the most in vitro active antimicrobial agent against P. aeruginosa (33.33%), followed by Azithromycin (39.58%). On the other hand multidrug resistance of P. aeruginosa was ranging between 11-16 different antibiotics, so that P.aeruginosa classified in to 2 groups according to number of resisted antibiotics; group B was the predominant group in this study. Result of sensitivity test agree, with minimum inhibitory concentration (MIC) ratio for, Augmentin, Cefixime, Pipracillin and Cefepime when they were 2-1024µg/ml, 32-1024µg/ml, 64-1024µg/ml, 16-1024µg/ml respectively. P. aeruginosa was able to produce number of virulence factors that associated with its pathogencity, 79.16% of isolates were able to produce β-lactamas ,further more able to produce Protease (89,58%),Lipase (87.5%), while 87.5% exhibited ability to adhesion to epithelial cells, 79.16% produce lecithinase and 72.91% were pyocyanin producer. our study demonstrated, High prevalence of nosocomial infections associated with the presence of multidrug resistant P.aeruginosa, especially in burn care unit.

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