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Antimicrobial Susceptibility Patterns Against Escherichia Coli and Prevalence of Extended–Spectrum β-Lactamases | ||
| Kerbala Journal of Medicine | ||
| Article 14, Volume 4, Issue 9, 2011, Pages 1019-1023 PDF (0 K) | ||
| Authors | ||
| Shrooq R. Kadhim; Afrah M. Hassan; Duaa S. Shoukat | ||
| Abstract | ||
| sixty seven samples were collected from urine, stool, pus, wounds of the patients and operating theater from Baghdad Hospitals in 2010. Sixty two isolates of Escherichia coli were diagnosed by bacteriological and biochemical tests. These isolates were submitted to hemolysis test and antibiotic sensitivity to 10 kinds of antibiotic disks on Mueller Hinton agar by disk diffusion method. The detection of β-lactamase production was also done as well as extended-spectrum beta-lactamase (ESBL) for all the isolates. Hemolysis test was positive for two isolates. All the isolates of E.coli were resistant to ampicillin and cephalothin (100%) and high resistance was observed to cephalixin (95.1%), tobramycin (90.3%), doxycycline (82.2. %) and nalidixic acid (70.9%). Both ciprofloxacin and nitrofurantoin was (67.7%). Low resistance was noticed to amikacin (11.2%) and trimethoprime (8.1%). β-lactamase test was positive for 57 isolates (91.9%), while three isolates (4.8%) showed positive result for extended-spectrum β-lactamase. Conclusion: Isolates of E.coli showed high resistance to ampicillin, cephalothin and cephalixin. Low resistance was revealed to amikacin and trimethoprime. Most of the isolates were positive for β-lactamase test (91.9%) and (4.8%) of the isolates were positive for extended-spectrum beta-lactamase. | ||
| Keywords | ||
| coli; Antimicrobial susceptibility; lactamase | ||
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