Contaminated with germs keyboard and mouse for computers used in units of the Central Health Laboratory (Baghdad), the study of resistance to anti-life and some disinfectants effect on bacterial isolates elected.

Sami Al-Janabi, Harith; Jassim Al-Karkhi, Kefah; Jassim Al-kelefawi, Essam

	Contaminated with germs keyboard and mouse for computers used in units of the Central Health Laboratory (Baghdad), the study of resistance to anti-life and some disinfectants effect on bacterial isolates elected.
CONTEMPORARY ISLAMIC STUDIENS MAGAZINE
Article 12, Volume 4, Issue 8, 2013, Pages 257-268 PDF (0 K)
Authors
Harith Sami al-Janabi; kefah Jassim al-Karkhi; Essam Jassim al-kelefawi
Abstract
In this study contaminated bacteria were isolated from Keyboard and Mouse of Computers using in Twenty one Units of Central Health Laboratory-Baghdad. The result of isolation was compared from Keyboard and Mouse by using T-test. The Pathogenic bacteria were examined for Antimicrobial activity for commonly used Antimicrobial using Disc diffusion method. The result of the recent study shows that the isolation percentage was 50% from Keyboard & 50% from mouse. The number of bacteria isolates were 193, 100 from Key board formed (52%) and 93 from Mouse formed (48%). The biggest number of bacteria isolated from Keyboard of Culture unit & AIDS unit. The lowest number of bacteria isolated from Keyboard of Immunity unit. No bacteria isolated from Keyboard of Hemorrhage unit, Hormones unit & Blood daises unit. The biggest number of bacteria isolated from mouse of AIDS unit. The lowest number of bacteria isolated from mouse of Clinical chemistry unit. No bacteria isolated from mouse of Hormones unit & Blood daises unit. The isolation from Keyboard & Mouse of Computers differs between Units. T-test analysis shows that the existence of significance differences (p<0.05) between number of bacteria isolated from Keyboard & Mouse. One hundred ninety three isolated were identified, eighty three were Normal Flora of Skin which was Staphylococcus epidermidis, formed 43% and 10 isolate was Diphtheroid spp. Formed 5% from all isolated. The remained isolated Bacteria 100 were Pathogenic Bacteria, included nine genus ((Bacillus subtilis 4 (2%), Enterococcus faecalis 7 (3%), Escherichia coli 11 (6%), Flavi oryzihabitons 5 (3%), Klebsiella pneumoniae 8 (4%), Micrococcus luteus 11 (7%), Pseudomonas aeruginosa 9 (5%), Staphylococcus aureus 38 (20%) & Streptococcus spp. 7 (3%). The Antimicrobial Susceptility test to commonly used Antimicrobial shows that the most isolate Bacteria were sensitive to Antimicrobial ( LEV 5, IPM 10, T 30, SXT 25) and Resistance to ( AMC 30, CX 5, CL 30) with some exception. The Antimicrobial Susceptility test of Staphylococcus aureus to Oxacillin showed the predominant of Oxacillin-Sensitive S. aureus (OSSA) which form 74% whereas the percentage of Oxacillin -Resistant S. aureus (ORSA) was 26%. The Efficacy of three disinfectants on selected three Isolated Bacteria for two duration of disinfectant exposure shows the efficiency of Chlorine compared with the other disinfectants.

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