Isolation and identification of Listeria moncytogenes by PCR from some food sources in Erbil city

M. Alzubaidy, Zainab; Ismail Kakey, Shean

	Isolation and identification of Listeria moncytogenes by PCR from some food sources in Erbil city
Euphrates Journal of Agriculture Science
Article 1, Volume 5, Issue 3, 2013, Pages 338-350
Authors
Zainab M. Alzubaidy; Shean Ismail Kakey
Abstract
The present study was carried out to evaluate foods in local markets of Erbil and Koya cities; 225 food samples were examined for the presence of Listeria species particularly L. monocytogenes from October 2010 to March 2011. The studied samples included 70 samples of raw poultry meat, 55 samples of raw milk, 50 samples of cheese and 50 samples of raw red meat. According to motility test, hemolysin production , sugar fermentation test, and PCR technique by using specific primer, 8 isolates of L. monocytogenes were identified, 1 (2%) isolate from cheese , 2 (4%) isolates from red meat and 5 (7.3%) isolates from chicken meat. The percentage of contaminated foods for Listeria species from chicken meat, raw milk, cheese and red meat were 20, 9, 20 and 22% respectively. The ability of L. monocytogenes to produce some enzymes which have role in pathogenicity of the bacterium like catalse, hippurate, hemolysin, protease, lipase , lecithinase, DNase, esterase, were determined, generally it was found that all the 8 isolates of L. monocytogenes were (100%) positive for catalase and hippurate hydrolysis, 6 isolates (75%) produce DNase and hemolysin ,7 isolates (87%) produce lipase and lecithinase and 5 isolates (61%) produced protease and esterase. Keyword: Listeria moncytogenes, PCR identification

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