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Effect of hydrogen peroxide and saturated animal fats on blood lipids level of adult male rats | ||
| Journal of Education and Science | ||
| Article 16, Volume 18, Issue 2, 2006, Pages 20-41 PDF (0 K) | ||
| DOI: 10.33899/edusj.2006.79295 | ||
| Authors | ||
| Khalid H. Shareef; Maan S. Kallo | ||
| Abstract | ||
| Thirty two albino male adult healthy rats were used as a biological and experimental model for this study, their body weight ranged between 250-275g and aged 3-4 months. They were divided according to the body weight into four groups. The first group considered to be control group, feed ordinary diet and normal drinking water, the second group feed ordinary diet and giving 0.5% H2O2 in freshly prepared drinking water, which contains oxygen and hydroxyl free radicals. The third group feed atherogenic diet (normal diet containing 4.69% saturated animal fats and 0.26% cholesterol) and normal drinking water, and the fourth group feed atherogenic diet and 0.5% HjC^ in drinking water for 30days experimental period. Nutritional status (body mass, afford diet, eliminated feces, liver, heart, kidney weights and diet lipid absorption) were estimated. Serum total lipid, cholesterol, triglycerides, phospholipids and various lipoproteins were determined, atherogenic indices were calculated and the malondialdehyde levels of liver, heart, kidney tissues were estimated. Analysis of variance and Duncan multiple tests showed the highly effect of 0.5% HsOz with/without saturated animal fats and cholesterol, was caused a decrease in body mass, ingesting diet weights and body absorbable lipid and increase values of serum total lipids, cholesterol, triglyceride, very low density and low density lipoproteins, In addition, it caused an increase values of atherogenic indices and a decrease in high density lipoprotein. Also the atherogenic diet alone was caused a similar affect of hydrogen peroxide on rat's nutritional status and serum lipid profile, therefore, the interaction affect of those two agents together was caused increase values of rat serum lipids, lipoproteins, malondialdehyde and a decrease in high density lipoprotein value. | ||
| Keywords | ||
| hydrogen peroxide; saturated animal fats; blood lipids level | ||
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