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Study of Lavandula officinalis L. buds of flowers extracts activity against some species of multi-drug resistant clinical isolates of bacteria | ||
| Iraqi Journal of Biotechnology | ||
| Article 1, Volume 12, Issue 2, 2013, Pages 82-91 | ||
| Authors | ||
| Asmma E. Al-Niaame; Raghad Akram Aziz | ||
| Abstract | ||
| Abstract: The aims of this study were to determine chemical composition and Qualitative phytochemical analysis as well as antibacterial activity of Lavandula officinalis L. flower bud extracts on the growth of some species of pathogenic bacteria. Results showed that percentages of crude proteins, crude fiber, total minerals ash, crude fats, total carbohydrates were 6.34, 8.94, 9.2, 6.71 and71.05% respectively . Results in ug/g showed that copper, lead, magnesium and iron values were 0.1141, 0.0077, 4.2003, 4.1330 respectively while cadmium and cobalt were not detected. The Qualitative Phytochemical analysis of methanol extracts revealed many bioactive chemical constituents including tanninis, alkaloids, saponins, flavonoids, coumarins, terpenoids, phenol, and the absence of steroids. The presence of these phytochemicals may justify the observed antibacterial activities in the current study. The antibacterial activity of the aqueous, methanol and extracts of L. officinalis against some pathogenic bacterial strains viz Acinetobacter baumannii, Enterococcus faecalis and Enterococcus faecium by employing well diffusion techniques and Minimum inhibitory concentration (MIC) assay. Methanol extract showed the highest inhibition zone (15-20) mm at 200mg/ml concentration, but using aqueous extract there was no of inhibition zone was observed with any organism. MIC of 25mg/ml was found for the methanol extract against all bacteria tested compared to the control indicating the presence of broad spectrum antibacterial compounds. The presence of these compounds in L.officinalis corroborates the antimicrobial activities against resistant bacterial strains. It is concluded that L. officinalis could be a potential source of active antimicrobial agents. | ||
| Keywords | ||
| Lavandula officinalis; antibacterial activity; multi; drug resistant; Qualitative phytochemical analysis; Methanol extract | ||
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