Detect the ability of bacteria isolated from gingivitis  to form biofilm

	Detect the ability of bacteria isolated from gingivitis to form biofilm
journal of kerbala university
Article 1, Volume 9, Issue 1, 2013, Pages 276-282 PDF (0 K)
Abstract
In this study , it had been collected 28 swab from patient suffering a gingivitis , then a biochemical test for identification these isolates were performed according to Bergy's manual, 1994. The results showed appearance of two groups of microorganisms which were bacteria and yeast. The five samples of yeast were neglected in this study and depend just on bacteria, which appear two groups according to Gram's stain. A gram negative bacteria( 2 isolates of Campylobacter and 4 isolates of Nisserosis) and gram positive bacteria( 10 isolates of Lactobacillus , 2 isolates of Streptococcus and 5 isolates Staphylococcus). Also it had been performed an antibiotic sensitivity test of a local antibiotic used to treat the patient suffering from gingivitis ,these were ( tetracycline, vancomycin, clindamycin, ampicillin and chloramphenicol) by following spread on agar method . The result showed that gram positive bacteria were more sensitive ( Lactobacillus , Streptococcus and Staphylococcus) than gram egative bacteria ( Campylobacter and Nisserosis) also it had been showed often isolates were resistance to ampicilline except the isolates no.(A5,A6,A8,A12,A13 and A20). Finally it had been detect the ability of these bacteria to form a biofilm according to tube method , which the results were 4 isolates out of 23 showed the ability to produce a strong biofilm, 11 isolates were moderate production of biofilm , 1 isolate was weak for production of biofilm while the rest did not have the ability to produce the biofilm.

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