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Effect of Tobacco Nicotiana tabacum L. Water Extracts in Growth and Regeneration Lactuca sativa L. leaves Callus and Isolation Nicotine from it | ||
| Rafidain Journal of Science | ||
| Article 3, Volume 24, Issue 12, 2013, Pages 31-54 PDF (0 K) | ||
| DOI: 10.33899/rjs.2013.80267 | ||
| Author | ||
| Firas H. Khathayer | ||
| Abstract | ||
| The present study aimed to show the role of variable concentrations of tobacco water extract (10،50،100،150) in initiation, growth and regeneration of leaves callus of lettuce (lactuca sativa L) plant by using MS 1.0mg/L of BA and NAA. The best medium was (MS + 1.0 mg/L BA and NAA +50mg/ml of tobacco extract), in which the fresh weight of callus reached 2.150 mg after 35 days of culture and with regeneration rate reached 63%. Also the result referred that nucleic acid (DNA, RNA), proteins and carbohydrate were greatly affected by tobacco water extracts and it varied with extract concentrations. As to carbohydrate content was varied with the increase or decrease in protein and nucleic acid content. The Detection and isolation of Alkaloid nicotine from the alcoholic extraction of lettuce were analyzed by (IR) where exhibited activity nicotine as bands. | ||
| Keywords | ||
| Tissue culture; callus; tobacco extract; nicotine isolation | ||
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