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Detection of Biofilm Formation in some Pathogenic Bacteria Using Tube and Congo Red Agar Methods | ||
| Rafidain Journal of Science | ||
| Article 4, Volume 24, Issue 12, 2013, Pages 55-65 PDF (0 K) | ||
| DOI: 10.33899/rjs.2013.80268 | ||
| Authors | ||
| Waad M. Raoof; Amera M. Mohamad; Aisha W. Al-Omari | ||
| Abstract | ||
| The research included studying the ability of some bacteria that were isolated from urinary catheter tips and urine samples in a previous study, to form biofilms by Congo red and tube method. The results showed that the highest biofilm formation using tube method was occurred by Citrobacter freundii, Escherichia coli and klebsiella spp.(100%), while Pseudomonas fluorescens did not form a biofilm. of gram positive bacteria, Staphylococcus aureus showed the highest biofilm formation (87.5%), while Micrococcus spp. showed the lowest level (33.3%). Using the congo red method in the gram positive bacteria, S.aureus showed biofilm formation of 31.25%, while S.epidermidis and Micrococcus spp. did not form biofilms. Using the same method for gram negative bacteria, E.coli showed a highest ability of (50%), while Ps.fluorescens and C.freundii did not form biofilms. | ||
| Keywords | ||
| biofilm; tube method; congo red method | ||
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