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Assessment of the Immune Response using ND Clone 30 Vaccine through Eye Drop and Drinking Water in Ross 308 and Cobb 500 | ||
| Al-Anbar Journal of Veterinary Sciences | ||
| Article 1, Volume 6, Issue 1, 2013, Pages 137-142 PDF (163.29 K) | ||
| Author | ||
| A. I. Ahmed | ||
| Abstract | ||
| The study was designed to assess the immune response of Newcastle disease (ND) Clone 30 vaccine in Ross 308 and Cobb 500 breeds. Day old chickens, 105 Ross and 105 Cobb which were housed together, dietary and environmental conditions were identical in each six experimental boxes (A: Ross-ED, B: Cobb-ED, C: Ross-DW,D: Cobb-DW, E: Ross control and F: Cobb-control) 35 chickens for each box, the group A and B were vaccinated with Clone 30 through eye drop only at 8th day,18 day and 28 day, while C and D groups were vaccinated with Clone 30 through eye drop at 8 day and drinking water at 18, 28 day, and control E and F groups remain without vaccination. ELISA test was used to evaluate the level of immunity in serum of chickens in all groups at 5, 16, 26 and 36 days of age, the results of the tests showed that there were high level of maternal antibody titer at 5 days of age persist until 16 day of age. No significant differences (P>0.05) among the vaccinated groups and control group but a significant differences at 16, 26 and 36 days old chickens vaccinated eye drop or drinking water compared with control, the result tests showed higher level of immunity in chickens of group A and B as compared with groups C, D both induced protective local mucosal immunity. No significant differences was detected between two breeds (p > 0.05). We concluded that eye drop route as individual vaccination program followed by drinking water route results in a good immune response and maintaining high antibody level to ND Virus in both breeds Ross and Cobb can be used in area were ND risks are not controlled. | ||
| Keywords | ||
| ELISA; Eye drop; Drinking Water; ND Clone; Ross and Cobb chickens | ||
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