Application of an electrometric method for measurement of blood cholinesterase activities in cats

A. Kata, A.; S. Alias, A.

	Application of an electrometric method for measurement of blood cholinesterase activities in cats
Al-Anbar Journal of Veterinary Sciences
Article 1, Volume 6, Issue 1, 2013, Pages 230-241 PDF (168.61 K)
Authors
A. A. Kata; A. S. Alias
Abstract
The aim of the present study is to detected for the accuracy of the way Electrometric method which used in the measurement of cholinesterase activity in the blood of untreated adult male cats. To serve these values a basis for comparison with the values of cholinesterase activity for cats that are exposed to organophosphorus pesticides and carbamate in the natural environment. Electrometric method applied to measure the normal activity of the cholinesterase in blood of adult male cats, highest cholinesterase activity recorded (the change in the function acidic 30 minutes) in the red blood cell 0.63, then the general blood 0.49 and the least in the blood plasma 0.37. Used cholinesterase activity in the red blood cell as a model to determine the linear relationship between the sample size of red blood cell and the activity of the cholinesterase, sample size ranged between (0.05 to 0.2 ml) was the best size of the sample 0.2 ml to measure activity of cholinesterase in the red blood cell. Cholinesterase activity used in the red blood cells as a model to determine the linear relationship between the time of incubate and activity of cholinesterase, 30 minutes was the best time of the incubate and the size of the 0.2 ml sample to measure the activity of cholinesterase the in red blood cells. Addition of Acetic acid (50 µmol/ml) to the reaction mixture of erythrocytes and Homogenized brain tissue linearly and gradually decreased the pH between the values 8 and 7. Recorded a significant difference in the cholinesterase activity, in red blood cells when comparing between acetylthiocholine iodide and acetylcholine iodide as substrates. Did not notice any significant change in the activity of the yeast with the use of both heparin and EDTA the anticoagulant. To illustrate the accuracy of the way Electrometric method was the coefficient of variation in plasma, red blood cells and whole blood (18.1, 11.2, 12.1)%, respectively when using 0.1 ml acetylcholine iodide as the basis of 7.5% in the reaction mix for the current study. Quinidine sulfate used to inhibition pseudo choinesterase ctivity in the red blood cell and plasma for male adult cats the percentage of pseudo choinesterase ctivity (12, 9.1)%, respectively, while the true cholinesterase activity is (88, 90.9)%, respectively. These results indicate that the used of modified electrometric method for measurement of ChE activities in adult male cats is simple, precise and efficient.
Keywords
cholinesterase; male cat adult; modified electrometric method

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