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Antibiotic Susceptibility Pattern And MecA Gene Detection In Methicillin Resistance Staphylococcus Aureus (MRSA) Isolated From Burn And Wound In Al-Diwaniya City. | ||
| Journal of University of Babylon | ||
| Article 1, Volume 21, Issue 3, 2013, Pages 917-926 | ||
| Author | ||
| Ibtisam Habeeb Said Al-Azawi | ||
| Abstract | ||
| This study was designed to detect the susceptibility of methicillin resistant staphylococcus aureus (MRSA) toward a group of antibiotic . Thirty six isolates were S . aureus were isolated from 157 clinical samples (burn and wound in Al-Diwaniya city) collected from Al-Diwaniya Teaching Hospital. Isolates were identified by traditional biochemical tests, then confirmed by VITEK-2 compact system. Antibiotic susceptibility was performed by disc diffusion method and minimum inhitory concentration(MIC) testing was done using VITEK-2. The presence of mecA gene was detected by conventional PCR technique. The result showed that 36 isolates(22.9%) were found as S.aureus. Eight isolates (22.2%) wereMRSA. All isolates 8/8 (100%) were resistant to penicillin, cefoxitin and methicillin, while moderate resistant 3/8 (37.5%) to vancomycin, erythromycin and rifampicin, lowresistant 2/8 (25%) to erythromycin and tetracycline. PCR assay revealed that 6 isolates harbored mecA gene (4 burn and 2 wound). | ||
| Keywords | ||
| antibiotic; MRSA; Burn; Wound; mecA gene | ||
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