The Inhibition  Effect of Paliurus  Spina-Christi Methanolic  Extract  on Trichophyton Mentagrophytes Growth  and Tri M4 Gene

T.Ahmed, Luma; W.AL-Sammarie, Khulood; KH.AL-Ani, Nabel; M.Jaber, Basim

	The Inhibition Effect of Paliurus Spina-Christi Methanolic Extract on Trichophyton Mentagrophytes Growth and Tri M4 Gene
Diyala Journal of Medicine
Article 1, Volume 4, Issue 1, 2013, Pages 101-109
Authors
Luma T.Ahmed; Khulood W.AL-Sammarie; Nabel KH.AL-Ani; Basim M.Jaber
Abstract
Back ground: Paliurus spina-christi methanolic extract is rich of phenolic compounds as well as other biological active compounds (sugars ,esters, fatty acids and alkaloids) . Aim:To asses that Paliurus spina-christi a good anti dermatophytic activity (Trichophyton mentagrophytes) . Materials and methods: five different concentrations of methanolic extract of P. spina-christi against T. mentagrophytes followed by the RNA extraction of Ttichophyton mentagrophytes grown in media containing P. spina-christi methanolic extract followed by complementary DNA synthesis to examine the expression of Tri m4 gene . Results: Statistical analysis revealed that at concentrations(25 and 50)µg/ml the differences between means of the growth diameters are non-significant (P≥ 0.05) as compared with the control while at concentrations (75,100,125)µg/ml the effect of methanolic extract on diameter growth was statistically significant (P≤0.05). Maximum growth inhibition was at 125µg/ml while minimum inhibition was at concentration 25 µg/ml .The percentage of inhibition was statistically significant especially at concentration 125 µg/ml it recorded 82%. The expression of Tri m4 gene was identified in presence of extract . Conclusion :Methanolic extract of P. spina-christi didn’t affect the expression of Tri m4 gene.
Keywords
Paliurus spina; christi; active compounds; Trichophyton mentagrophytes; Tri m

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