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E. coli Response under Chemical Stress: An Experience with Amikacin, Gentamicin and Ciprofloxacin | ||
| Al-Mustansiriyah Journal of Science | ||
| Article 1, Volume 24, Issue 4, 2013, Pages 15-22 | ||
| Author | ||
| Batool Hazim Abdulwhab | ||
| Abstract | ||
| Chemical stress caused by toxic and harmful compounds may facilitate bacterial transition from free-living organisms to a host invading pathogens. This study aimed to investigate the susceptibility of E. coli to aminoglycosides (gentamicin and amikacin) and fluoroquinolone (ciprofloxacin) when chemically stressed with triton X-100 or hydrogen peroxide (H2O2). E. coli isolated from urine and stool samples were studied. The isolates were cultured in Muller Hinton broth and incubated either with H2O2 (10mM) or Triton X-100 (0.05%) in the presence or absence of one of the following antibiotics: gentamicin, amikacin, and ciprofloxacin of concentrations from 0.0312 to 32 µg/ml at 37ºC . Co-administration of ciprofloxacin with either H2O2 or Triton X-100 improve the susceptibility of E. coli at MIC range from ≥ 1 to 32 µg/ml. The addition of H2O2 improved the susceptibility of E. coli to amikacin and gentamicin. Adaptive response of E.coli was observed in one isolate incubated with triton X-100 and gentamicin. It concludes that Triton X-100 induces chemical stress in E.coli treated with gentamicin while H2O2 produces synergism with aminoglycosides | ||
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