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Isolation, Diagnosis And Extraction Of Outer Membrane Proteins Of Helicobacter Pylori Bacteria | ||
| Al-Mustansiriyah Journal of Science | ||
| Article 1, Volume 24, Issue 4, 2013, Pages 23-30 | ||
| Author | ||
| Rajwa Hassan Essa | ||
| Abstract | ||
| This study included 92 patients suffering from dyspepsia , gastritis , duodenal ulcer , and gastric cancer , 55 biopsy were collected from patients gave positive test with Rapid anti H. pylori test , their age ranged between (17-76) years. The specimens were colected from AL-Kadhymia teaching hospital during the period from December 2011 to April 2012 . Microearophilic , gram negative Helicobacter pylori were isolated and identified using different morphological and biochemical test such as catalase, oxidase and urease test . Outer membrane proteins were extracted from these bacteria using Murphy method and the concentration of proteins were evaluated by spectrophotometer , then proteins detection and the molecular weight of proteins were evaluated by electrophoresis. Result revealed that ten bands with must renged between (16.914 – 340.476) KDa were present and the concentration of protein was (2.76028) mg/ml | ||
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