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Pathogenesis of Providencia rettgeri in mice | ||
| Journal of University of Babylon | ||
| Article 1, Volume 21, Issue 8, 2013, Pages 2785-2800 | ||
| Authors | ||
| Hayder S.Obayes; Frias GAbd | ||
| Abstract | ||
| Providencia rettgeri strains were isolated from urinary tract infections (UTIs) patients and identified according to morphological and biochemical tests, furthermore, the identification was confirmed using the Hi 25 Enterobacteriaceae system. The crude lipopolysaccharide (LPS) was extracted from P.rettgeri isolate by sonication with ethylene diamine tetraacetic acid (EDTA) and then with enzymic digestion, then partially purified by an ion exchange chromatography .It have been revealed that the lethal dose 50% (LD50) of P.rettgeri was about 5.62 × 107 cell/mouse . The microscopic examination of stained tissue sections of organs obtained from mice injected with sub lethal doses of P.rettgeri and LPS, showed sever changes in kidney and bladder induced by bacterial suspension than LPS, while sever changes were shown in spleen ,liver , and intestine caused by LPS compared with control animals. In general, these histopathological changes included the congestion, hemorrhage, migration and infiltration of inflammatory cells, tissue necrosis and oedema but in spleen there were hypertrophy of white bulb and congestion of red bulb . | ||
| Keywords | ||
| Pathogenesis; LPS; Providencia; histopathogenesis; mice | ||
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