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Effects of some compounds on the A-Amylase Activity Purified From Abena-24 Wheat Flour Treated by TriboliumConfusum | ||
| Journal of Education and Science | ||
| Article 6, Volume 17, Issue 2, 2005, Pages 50-56 PDF (0 K) | ||
| DOI: 10.33899/edusj.2005.81314 | ||
| Author | ||
| M. K. Ismail | ||
| Abstract | ||
| a-Ainylase was purified from Abena-24 wheat flour treated by Triboliumconfusuminsect using heat treatment, ammonium sulphateprecipitation, dialysis, and anion exchange chromatography. Three a-amylase isoenzymes were isolated (I, II and III) with elution volume of 90, 170 and 220 ml respectively, with specific activity of 73000, 185818 and 462333 mu/mg protein, respectively compared with the crude enzyme. Many compounds showed different effects on the a-amylase isoenzymes activity. Pheriyl .hydrazine, EDTA and HC1 showed an inhibitory effect between (70-80%), (49-53.5%) and (46.4-67.5%) respectively. Using heavy metales , it was observed that Cu+2 caused an inhibitory effect between (32.8-42,2%>), while Fe+2 caused an inhibitory effect between (26.3-53.5%). On the other hand, no significant inhibitory effect was shown with iodoacetamide and tristearin. Chloride ion and calcium ion showed an activitory effects on the a-amylase isoenzymes 1, II and III ranging between. (27-45.4%) for CI" and (15-82%) for Ca+J | ||
| Keywords | ||
| Abena-24 wheat flour; Triboliumconfusuminsect; ammonium sulphate precipitation | ||
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