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Preparation and Purification of Immunoglobulin Y from Egg Yolk of Immunized Hens | ||
| Journal of Univesity of Thi-Qar | ||
| Article 1, Volume 8, Issue 2, 2013, Pages 185-194 | ||
| Author | ||
| Ali A. Al-Edany | ||
| Abstract | ||
| Two purification methods of egg yolk immunoglobulin (IgY) based on precipitation using charcoal-PEG and dextran-PEG were developed. These methods were com- pared with chloroform extraction and polyethylene glycol (PEG) precipitation methods. The results showed that protein contents were high with chloroform method (12.8 mg/ml) followed by dextran-PEG (10.5 mg/ml), charcoal-PEG (8.13 mg/ml) and PEG method (4.4 mg/ml). The purity of resultant IgY was homogeneous with dextran-PEG method followed by charcoal-PEG method, less purity was in PEG method followed by chloroform extraction method. | ||
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