Phenotypic and Genotypic (mecA gene) Characterization of Borderline Oxacillin Resistant Staphylococcus aureus (BORSA) Isolated in Al-Hilla City

H. Al-Safaar, Venus; H. Al-Charrakh, Alaa

	Phenotypic and Genotypic (mecA gene) Characterization of Borderline Oxacillin Resistant Staphylococcus aureus (BORSA) Isolated in Al-Hilla City
Medical Journal of Babylon
Article 1, Volume 10, Issue 4, 2013, Pages 956-968
Authors
Venus H. Al-Safaar; Alaa H. Al-Charrakh
Abstract
Out of 130 clinical samples, a total of 37 (28.4%) Staphylococcus aureus isolates were recovered, from skin samples, ear, blood and throat, and both urine and vagina as (75.6), (8.1%), (5.4%), and (10.8%), respectively. Results showed that 21 (56.7%) isolates were identified as β-lactam resistant. The susceptibility to 25 antibiotics were tested using disc diffusion test resulting in 80% of 21 bacterial isolates were resistant to 22 antibiotics but they were sensitive to teicoplanin, netilmicin and chloramphenicol. For detection of Borderline oxacillin resistance S.aureus (BORSA), all of 21 isolates were tested by using oxacillin disc (1mcg). Results showed that all 21 isolates were resistant to oxacillin and identified as MRSA as they were resistant to both oxacillin and cefoxitin. Two–fold agar dilution susceptibility method to oxacillin and vancomycin were performed for 21 isolates. All 21 isolates showed resistance to oxacillin, and 16 (%76) were sensitive to vancomycin while 5 (%24) decreased susceptibility to vancomycin (VISA). All 21 isolates had mecA gene and identified as MRSA and no BORSA detected in this study which lack mecA gene.

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