Effect of Crude Oil of Black Seeds (Nigella sativa) on White Blood Cell and Hematocrit of Male Albino Mice Treated with Low Toxic Dose of Paracetamol

Hadi Kamil, Zainab

	Effect of Crude Oil of Black Seeds (Nigella sativa) on White Blood Cell and Hematocrit of Male Albino Mice Treated with Low Toxic Dose of Paracetamol
Medical Journal of Babylon
Article 1, Volume 10, Issue 4, 2013, Pages 1005-1012
Author
Zainab Hadi Kamil
Abstract
The study was carried out to investigate the effect of crude oil of black seeds (Nigella sativa) on white blood cell and hematocrit of mice treated with low toxic dose of paracetamol. The study included twenty (20) adult male mice Balb/c randomly divided into four groups. Group1 was injected intra peritoneal with 300 mg / kg body weight (B.W.) of paracetamol which considered as low toxic dose, followed by 0.3 ml of normal saline(0.9 % w/v) was administrated orally . Group2; mice administrated orally with 0.3 ml of crude traditional oil of Nigella sativa, then injected intra peritoneal with 0.3 ml of normal saline(0.9 % w/v). Group3, mice injected intra peritoneal with 300 mg / kg (B.W.) of paracetamol , then administrated orally with 0.3 ml of crude traditional oil of Nigella sativa . Group4 the control group, mice injected intra peritoneal with with 0.3 ml of normal saline (0.9 % w/v), then administrated orally again with 0.3 ml of normal saline. The experimental time of treatment were 24 hours for all groups. The total leukocyte counts (TLC), differential lecukocyte count (DLC) and hematocrit (Hct) was determined. Statistical analysis of data demonstrated significant increase (p<0.05) in TLC, lymphocyte count, neutrophils %, neutrophils count and lymphocyte count of mice treated with Nigella sativa crude oil only and with (300 mg/kg) B.W of low toxic dose of paracetamol groups. Treatment with low toxic paracetamol (300mg/kg) and crude oil of Nigella sativa show no significant changes in hematocrit (Hct) value in mice between the four groups.

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