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PCR detection of rmpA gene among Klebsiella isolated from wound and burn infections | ||
| Magazine of Al-Kufa University for Biology | ||
| Article 1, Volume 5, Issue 2, 2013, Pages 124-129 | ||
| Authors | ||
| Ruaa Sh. Al-Rediany; Abbas Sh. Al- Muhanna | ||
| Abstract | ||
| One hundred and twenty five Gram-negative-lactose fermented bacteria grown on MacConkey agar were collected from burn and, wound infections, in Al- sadar Medical City in Al- Najaf province. The bacterial isolates were identified according to cultural characteristics ,the results revealed that 40(32%) isolates were identified as Klebsiella spp, all of them 40(100%) isolates were belong to K. pneumonia subsp. pneumonia .The capsule of klebsiella isolates were tested .the result revealed all Klebsiella isolates40(100%) were capsulated ,the gene coding capsule in isolates gave positive results. Genotypically were studied carefully using PCR technique , the results pointed out that 35 (87.5%) of bacterial isolated have rmpA gene coding for producing polysaccharide capsule . | ||
| Keywords | ||
| Gram; Negative; lactose fermented; klebsiella; PCR technique; pcr | ||
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