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Extraction and partial purification of antimicrobial gent produced by Streptomyces spp. in Babylon province | ||
| Journal of University of Babylon | ||
| Article 1, Volume 22, Issue 1, 2014, Pages 279-284 | ||
| Authors | ||
| Samer M.Al-Hulu; Eman M.Jarallah; Alaa H. Al-Charrakh | ||
| Abstract | ||
| The Strptomyces was isolated from soil samples and identified as Streptomyces spp. The cultural characteristics of Streptomyces spp isolates showed grey aerial mycelium and was yellowish brown to green substrate mycelium with no pigmentation on YMD agar medium, and no melanin production.. The active metabolite was extracted using ethyl acetate (1:1, v:v).The crude extract showed high antibacterial activity against S.aureus with 17 mm inhibition zone. The UV spectrum of antibacterial agent showed a single peak with maximum absorption( λ max) at 285 nm . The IR (KBr) spectrum exhibited a peak at position 3400 cm-1 which indicate presence of hydroxyl (OH) group , and anther peak at 2800-3000 cm-1 which refer to presence of C-H alphatic and aromatic group and in addition to third peak at 1760 cm-1 that indicates to presence of carbonyl group (C=O). | ||
| Keywords | ||
| KEYWORDS; antimicrobial; Extraction and purification; Iraq | ||
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