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Isolation of Multi Antibiotics Resistance Escherichia coli from urinary tract infection and the Detection of PapC and fimH virulence genes by Polymerase chain reaction Technique | ||
| Diyala Journal For Pure Science | ||
| Article 1, Volume 10, Issue 1, 2014, Pages 112-127 | ||
| Authors | ||
| Shaymaa K.Abass; ; im R. Ali; Sawsan H.Authman | ||
| Abstract | ||
| One hundred fifty urine samples were collected from urinary tract infections. patient ages is ranging with two days to 73 years, 92 females , 47 males and 11 cases of children for both sex. A total of 65 isolates of E. coli. Drug sensitivity of all E. coli isolates was evaluated using a disc diffusion method. Most E. coli strains showed variable resistance to different antibiotic ,showed high degree of sensitivity towored Nitrofurantoin Ciprofloxacin, Pifloxacin, and Norfloxacin showed the isolates Approximately 100% resistance to Amoxicillin/clavulanic acid and Cloxacillin in all tested isolates. And local isolate showed multidrug resistance range from (28% - 100% ). The expression of adhesion factor type P fimbriae (CAFl, CAFΠ, CAFШ) was determined as followed (89%, 54% , 55%) respectively .As well as adherence of E. coli isolates to uroepithelial cells is showed 100% in the local isolates . A total of 28 UPEC strains were selected for genotypic characterization of virulence factor gene ( fimH and papC) by using PCR to detect the relation between phenotype determine with present gene in all of these isolate . The results indicated that the occurrence of fimH was detected in (71%) of isolates ,while pap C was detected in (79%) of isolates .These results demonstrated the variable phenotypic expression when compared to genotypic detection . | ||
| Keywords | ||
| coli; UPEC; Virulence genes; pap C; fim H; PCR; multi; Antibiotic resistance; UTI | ||
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