- All Journals
- Al-Qadisiyah University
- Basrah University
- Kirkuk University
- Mosul University
- The Iraqi Borad for Medical Specialization
- University of Anbar
- University of Technology
- Al Mustafa University
- Al-Zahraa University for Women
- Alnoor University
- Kunooz University
- Misan University
- Ninevah University
- Tikrit University
- University of Al-Hamdaniya
- University of Kerbala
Comparison between Recombinant Immunoblot assay 3rd generation and enzyme linked immunosorbent assay for detection hepatitis C virus . | ||
| Journal of University of Anbar for Pure Science | ||
| Article 10, Volume 7, Issue 1, 2013, Pages 36-41 PDF (334.51 K) | ||
| Document Type: Research Paper | ||
| DOI: 10.37652/juaps.2013.83124 | ||
| Authors | ||
| Hanaa Naji Abdullah1; Mohammed A. Saleh2; Khalid J. Khaleel3 | ||
| 1Health and medical technology college, Foundation of technical education. | ||
| 2Diyala University - College of Science . | ||
| 3Iraqi center for cancer researches , Al-Mustansryiah University. | ||
| Abstract | ||
| This study was carried out to compare between recombinant immunoblot assay 3rd generation and enzyme linked immunosorbent assay for identify hepatitis C virus. Seventy six Iraqi patients undergo hepatitis C were involved in this study. The study was carried out from July 2010 to April 2011 were followed up in public health center- Baghdad. These patients included 54 males and 22 females as well as their ages ranged between (8-68) year with a mean age of 47.1±13.79, besides, the majority of patients are at the age between 31-60 year (54 %), while children elicit less frequency of infection (2 %). Additionally the ratio between male to female was 2.45:1. There are two methods which are used for HCV diagnosis. The first method by using ELISA technique for detection of anti HCV antibody. 61 out of 76 sera samples of hepatitis patients (i.e. 80.2%) were found to be positive for this test. Another advanced method such as recombinant immunoblot assay 3rd generation (RIBA) has been applied, all these specimens gave positive results (100 %) with significant difference was noticed between them (P<0.05), therefore the current results confirm that a RIBA 3rd generation is more sensitive manner to detect hepatitis C virus than ELISA. | ||
| Keywords | ||
| Immunoblot assay; Immunosorbent assay; Hepatitis c virus | ||
| References | ||
|
1- Beeck, A. and Dubuisson, J. (2003). Topology of hepatitis C virus envelope glycoproteins. Rev. Med. Virol. 13 (4): 233–41.
2- Caruntu, F.A. and Benea , L. (2006). Acute hepatitis C virus infection: Diagnosis, pathogenesis, treatment. J. of Gastrointes. and Liver Dis.;15 (3): 249–56.
3- Gordon, S.C. ; Bayati, N. and Silverman, A.(1998). Clinical outcome of hepatitis C as a function of mode of transmission. Hepatol.; 28:562.
4- Gretch, D.R. (1997) . Diagnostic tests for hepatitis C. Hepatol.; 26 (3): 43S-47S.
5- Shev, S.; Forberg ,A. and Fryden, S.(1993). 2nd generation hepatitis C ELISA antibody tests confirmed by the 4 antigen recombinant Immunoblot assay correlate well with hepatitis C viremia and chronic liver disease in Swedish blood donors. Vox Sang ; 65: 32-37.
6- Reddy, A.K.; Dakshinamurty, K.V. and Lakshmi, V. (2006).Utility of HCV core antigen ELISA in the screening for hepatitis C virus infection in patient on heamodialysis. Ind. J. Med. Microbiol.; 24: 55-57.
7- Berry, V.; Arora, R. and Paul, P.( 2005). Hepatitis C – Clinical outcome and diagnosis. Ind. J. K. sci.; 7(1): 129-32.
8- Banker, P.D. (2003) .Viral hepatitis. Ind. J. Med. Sci.;57: 461-68.
9- Damen, M.; Zoaijer, H. and Cuyper, H. (1995) . Reliability of the third-generation recombinant Immunoblot assay for hepatitis C virus. Transf.; 35: 745-749.
10-Anne, F.; Celona, M.C. and Macnish , P. (2004) Hepatitis C in Los Angeles public hepatitis clinic: Demographic and biochemical differences associated with Race-Ethnicity. Clin. Gastroenterol. Hepatol.; 2 :459-462.
11-Sabri ,H.J.A.(2003) .The diagnostic role of liver biopsy in grading ,staging and etiology of chronic hepatitis. A thesis fellow ship Iraq commission for Medical Specialization in Pathology .
12- Douraid, K.S. (1995).Prevalence of hepatitis C virus infection in a sample of Iraqi patients with chronic liver disease. A thesis fellow ship Iraqi commission for Medical Specialization in Medicine.
13- Derek, P. ; Norah, A.; Terraul, T. (2005).Cost –effectiveness of Hepato cellular carcinoma surveillance in patients with hepatitis C virus –related cirrhosis. Clini. gastro-entero. and Hepato.;3: 75-84.
14-Rahi, S.J. (2003) .Cytokines profile in Iraqi patients infected with HCV. M. SC. Thesis, College of Medicine, University of Baghdad .
15-Moatter, T.; Hussainy, A.S.; Hamid, S.; Ahmed, Z. and Siddiqui, S. (2002). Comparative analysis of viral titers and histologic features of Pakistani patients infected with hepatitis C virus type 3. Int. J. Infect. Dis.; 6: 272-276.
16- Vandelli, C.; Renzo, F.; Braun, H.B.; Tisminetzky, S. and Albrech, M. (1999) . Prediction of successful outcome in a randomized controlled trial of the long-term efficacy of Interferon alpha treatment for chronic hepatitis C. J. Med. Vir. 58:26-34.
17- Lau, G.K.; Davis, G.L.; Wu, S.P.; Gish, R.G.; Balart, L.A. and Lau, J.Y. (1996).Hepatic Expression of hepatitis C virus RNA in chronic Hepatitis C: A study by in situ Reverse-Transcription Polymerase Chain Reaction. Heaptol .; 23(6): 1318-1323.
18- Sun, D.X.; Zhang, F.G. and Geng, Y.Q. (1996).Hepatitis C virus transmission by cosmetic tattoing in women. Lancet ; 347: 541-42.
19- Dienstag, J.L.(1991). Viral hepatitis: A mini-symposium in which recognized experts critically review clinically relevant aspects of acute and chronic. Semin. Liver Dis.; 11:23.
20- Wang, H.; Li, B. and Li, Y.(2007). Study on the comparison of different methods in testing HCV antibodies and the relationship among anti-HCV,HCV RNA and ALT. J. Infect. Dis. ;8:14.
21- Qi, Z.; Zhou, C. and Yang, Z. (1999). Evaluation on the capabilities for detecting anti HCV core and NS3 antibodies with two anti HCV EIA kits of the third generation. J. of Microbio.and immunolo.; 5:67-71.
22-Dow ,B.C.; Munro, H.; Buchanan, I.; Follett, E.A. and Davidson, F.(1996). Third-generation recombinant immunoblot assay: comparison of reactivities according to hepatitis C virus genotype. Transf.; 36(6):547-51. | ||
|
Statistics Article View: 416 PDF Download: 322 |
||