Study The Resistance of some  Bacteria at the Most Commonoly Isolated from Tikrit Teaching Hospital Infections to Antibiotics and their Relation with Plasmids

A. Mohammed, Shaimaa; O.Mohammed, Khalid; M. Raoof, D; Waa,

	Study The Resistance of some Bacteria at the Most Commonoly Isolated from Tikrit Teaching Hospital Infections to Antibiotics and their Relation with Plasmids
Tikrit Journal of Pure Science
Article 1, Volume 18, Issue 2, 2013, Pages 92-107
Authors
Shaimaa A. Mohammed; Khalid O.Mohammed; d M. Raoof;
Abstract
Abstract One hundred fourti samples were collected from hospitalized patients for three days or more in the Tikrit Teaching Hospital during the period from 1/8/2009 until 1/12/2009 to determine the most commonly isolated bacterial from nosocomial infections in Tikrit Teaching Hospital and their resistance to antibiotics and to study the relationship between antibiotics resistance and plasmid content in the most commonly isolated bacteria and to evaluate the prevalence of these plasmids in the hospital environment . These samples include : (75) Urine , (28) stool , (28) of wound swabs and (9) sputum samples .The incidence of nosocomial infection was (45.7%) of them, urinary tract infections were (50.7%) from (75) samples, followed by wound infections (42.9%) from (28) samples , gastrointestinal infections (39.3%) from (28) samples then pneumonia (33.3%) from (9) samples the nosocomial infections were more common in age group 51-89 years.Different bacterial species were isolated in this study and the results showed that the most commonly isolated type was Citrobacter koseri in a proporation (21%) of urinary tract infections, followed by the following bacterial species: Escherichia coli (15.7%), Staphylococcus schleiferi (10.5%) , Staphylococcus epidermidis (7,8%), Proteus vulgaris, Staphylococcus saprophytica, Klebsiella ozaenae , Klebsiella ornithindytica in aproportion (5.2%) for each, Enterobacter aerogenes , Providencia stuartii , Staphylococcus warneri, Staphylococcus aureus, Proteus mirabilis in aproportion (2.6%) for each .Citro. koseri were also the most common in wound infections (25%), followed by the following bacterial species: E.coli (16.6%), P.mirabilis (16.6%), Staph.schleiferi , P.vulgaris, Prov.stuartii and Prov.rettgeri in aproportion (8.3%) for each.Citro.koseri were also the most common in the gastrointestinal tract infections in a proporation (36.3%) , followed by E.coli (18.1%), S.flexneri (18.1%), P.mirabilisc, Prov.stuartii, Prov.alcalifaciens in a proporation (9%) for each.. While Strept.pneumoniae was the most common (66.7%) in penemonia cases followed by K.pneumoniae (33.3%). The isolated bacteria were tested for antibiotics susceptibility and it has been found that most of these isolates showed high resistance to the studied antibiotics other than Agenus Staph.scleiferi was sensitive to Amikacin.Eleven of most commonly isolated bacteria which were multidrug resistant were chosen for the curing experiments using SDS to study the relationship of their plasmids with resistant to antibiotics. The results showed that the cured cells of E.coli lost their resistance to Tetracycline Ciprofloxacin and Cefotaxime which Citro.koseri lost their resistance to Tetracycline while K. ornithinolytica lost resistance to both antibiotics Tetracycline and Ciprofloxacin either P.mirabilis lost resistance only to Tetracyclin as well as Strept.pneumoniae lost their resistance to Cefotaxime , tracycline and Ampicillin. Which may mean that the genes responsible for resistance to these antibiotics are located on plasmids as proven by the results of electrophoresis. Electrophoresis results also showed that all studied bacterial isolates had the same plasmid profile which may regarded as an epidemiological pointer that plasmids are conjugative which have the ability to transfer antibiotic resistance to other bacterial strains of the same species or to other different bacterial species .

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