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Isolation and identification of SulfolobusSPP. From hot spring using classical and molecular methods in Hammam AL-Allel by nenavah governorate | ||
| Tikrit Journal of Pure Science | ||
| Article 1, Volume 18, Issue 2, 2013, Pages 26-33 | ||
| Authors | ||
| Mohammed Abdul-Razak Al-Qatan; Basima A. Abdullah; | ||
| Abstract | ||
| The isolates of Sulfolobus were identified depending on Microbiological characteristics as colour , consistency of colonies , in addition to biochemical characteristics of growing colonies . also the susceptibility of antibiotic tests, moreover to physiological and molecular characteristics. So two species were obtained : Sulfolobus acidocaldarius , Sulfolobus solfataricus . The result of antibiotic sensitivity of the species was in dictated their high resistance to all antibiotics under study , except Sulfolobus acidocaldarius which gave high sensitivity to Gentamicin . The isolates were indicated good growth at optimum temperature between (37-40)co , also isolates of SulfolobusSPP.gave growth at rang of temperature between (35-70) Co . When we cultivated the isolates at different level of PH the SulfolobusSPP. Were grown at PH between (3-7). And the best at (3-4). And when the isolates were cultivated at different level of sodium chloride , the species of SulfolobusSPP. Gave best growth at (2-3%) of NaCL. The PCR technique were done based on amplification of genomic DNA using special primer for Archaeal species which indicated distinct bands with molecular weight of 930-950 bp when they electrophrized on agaros gel . | ||
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