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The Study of some Plant Extracts Effect on Free Radicals Removal from DNA in Vetro | ||
| Engineering and Technology Journal | ||
| Article 1, Volume 31, 7B, 2013, Pages 174-180 PDF (560.52 K) | ||
| DOI: 10.30684/etj.31.7B.13 | ||
| Authors | ||
| Abdul Wahid B. Al-Shaibani; Esmaeel K. Shubber; Azhar M. Haleem | ||
| Abstract | ||
| The removal efficiency of Several plant extracts of Nagilla sativa linn, Allium sativum sativum and Allium sativum ophioscordon of free radicals from pure DNA molecule at concentration 5 µgm / ml have been studied when we added 1×10-5 M of H2O2 with plant extracts mixture by measuring absorbency at 260 nanometer . Breaking index for nitrogen base pair was calculated. The plant extracts showed high ability in free radicals scavenge when used in concentrations (0.1, 1, 10, 100) µgm / ml which effect in DNA molecule. Also the DNase activity from different plant extracts was measured in DNase agar. Alcoholic mixture activity of three plants in (1،10،100) µgm/ml concentration was studied by monitoring the protection effect on pBR322 plasmid DNA from Escherichia coli which was protect t pBR322 plasmid he plasmid DNA from breaking by H2O2. | ||
| Keywords | ||
| Free Radicals; DNA; Plant extracts; Medical Plants; pBR | ||
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