Immunohistochemical study of CD15 expression in CD30+ classical Hodgkin's lymphoma

Jawad Kadhum, Thaeer; Rassul Hussain, Aws; Jamal Naser, Nawar

	Immunohistochemical study of CD15 expression in CD30+ classical Hodgkin's lymphoma
Al-Qadisiah Medical Journal
Article 1, Volume 9, Issue 2, 2013, Pages 237-244 PDF (0 K)
Authors
Thaeer Jawad Kadhum; Aws Rassul Hussain; Nawar Jamal Naser
Abstract
Background: Hodgkin lymphoma (HL) is a histologically defined B-cell neoplasm that includes two distinct types of disease, classical and nodular lymphocyte-predominant. Aim of the study: To evaluate the immunohistochemical expression of CD15 in CD30+ classical Hodgkin’s lymphoma. Patients and Methods: This retrospective study included revision of 52 lymph nodes specimens from patients newly diagnosed with HL from January 2005 to September 2010. These samples were collected from Al-Yarmouk Teaching Hospital, Baghdad Medical City and Al-Atheer private laboratory. Only well-preserved samples that stood a retrospective confirmatory morphological revision and revealed IHC CD30 stain {from DAKO} positive reactivity were included in this study. All biopsies were formalin-fixed and paraffin embedded. A 4 micron sections obtained and stained with hematoxylin and eosin and CD15 monoclonal antibody {from DAKO} according to the kit-included instructions. Results: This study revealed that the CD15 positive classical HL are 24 (75%), 10 (100%), 6 (100%) and 0 (0%) for MC, NS, LD and LR respectively. Conclusions: All cases of cHD should undergo immunophenotypic analysis for CD15 in addition to CD30 antigen.

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