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In vitro effects of hypertonic saline solution on whole blood clotting | ||
| Kerbala Journal of Medicine | ||
| Article 1, Volume 6, Issue 1, 2013, Pages 1568-1572 PDF (0 K) | ||
| Author | ||
| Mohammed Shnain Ali | ||
| Abstract | ||
| background: hypertonic saline solution is frequently used to in the management of hemorrhagic shock. Objective: To determine the effects of hypertonic (7%) saline solution on whole blood coagulation (clotting) in vitro. Patients and methods: Ten healthy volunteers (5 males and 5 females with age range of 28- 44 years) with no previous medical history of bleeding tendency and no medical history of drug intake were studied after their consent. Hypertonic (7%) saline was prepared immediately before use. For each patient, 4 ml of venous blood sample was obtained and investigated as follows: Undiluted 450 µl of whole blood for baseline coagulation tests [prothrmbin Time (PT), activated partial thromboplastin time (APTT) and thrombin time (TT)]. After that, the same above tests were done after dilutions of whole blood (i.e whole blood replacement by hypertonic saline and isotonic saline for each dilution). An Isotonic (0.9%) saline dilution serves as control. Blood volume replacements at 2.5%, 5%, 7.5% and 10% were done and studied. Results: The baseline coagulation tests (PT, APTT, and TT) were normal for all 10 (100%) of subjects. At 2.5% and 5% of blood volume replacement PT, APTT and TT were slightly prolonged, but showed no statistically significant difference between hypertonic (7%) saline and the control (isotonic saline) while PT, APTT and TT were prolonged in hypertonic (7%) saline with statistically significant difference (p<0.05) from isotonic (0.9%) saline at 7.5% and 10% of whole blood volume replacement in 10, (100%) of subjects. Conclusion: The study showed that hypertonic (7%) saline disturbs coagulation in vitro when it replaces 7.5% or 10% of whole blood volume. | ||
| Keywords | ||
| Hypertonic saline; isotonic saline; blood clot | ||
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