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Effect of Sperm Selection by ''Swim-Up" Technique on the Sex Ratio of In Vitro Produced Ovine Embryos | ||
| The Iraqi Journal of Veterinary Medicine (IJVM) | ||
| Article 12, Volume 37, Issue 2, 2013, Pages 172-177 PDF (0 K) | ||
| Authors | ||
| Saja Hadi; Ihsan H. AL-Timimi | ||
| Abstract | ||
| The objective of this study was to investigate the percentage of male and female ovine embryos produced after oocytes fertilization by sperm selected with self-migration (swim-up) technique. The sex of in vitro produced embryos was determined by Polymerase chain reaction (PCR). Results indicate that the fertilization by sperm selected using Swim-up technique was 81.8% (9/11) male and 18.1% (2/11) female in vitro produced embryos when centrifuged at 200×g. While centrifugation at 300×g was 72.7% (8/11) male and 27.2% (3/11) female embryos. It was concluded that the use of “swim-up” technique refer to significant (P<0.05) occurred for increasing male embryos ratio compared with normal ratio (50%) which represent the sex, and it is for the first time, preselected sperms used for In Vitro fertilization (gender selection) in farm animals in Iraq. | ||
| Keywords | ||
| ovine; embryos; swim; Gender; PCR | ||
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