HLA Class I and II Genotyping by PCR-SSO in Patients with Type-1 Diabetes Mellitus

A. Al-Hassan, Ahmed; Mohammad, Anwar; H. Al-Ghurabi, Batool; Abdul Elah, Laith

	HLA Class I and II Genotyping by PCR-SSO in Patients with Type-1 Diabetes Mellitus
Iraqi Postgraduate Medical Journal
Article 1, Volume 12, Issue 4, 2013, Pages 519-524 PDF (0 K)
Authors
Ahmed A. Al-Hassan; Anwar Mohammad; Batool H. Al-Ghurabi; Laith Abdul Elah
Abstract
ABSTRACT: BACKGROUND: Type 1 diabetes mellitus is a chronic autoimmune disease that involves destruction of the pancreatic beta cells. It is well known that both genetic and environmental factors involved in pathogenesis of type-1diabetes mellitus. OBJECTIVE: This study seeks to determine whether there is any association between human leukocyte antigen class I and II alleles and type-1diabetes mellitus. SUBJECTS AND METHODS: Seventy type-1 diabetes mellitus patients compared to 30 apparently healthy individual were enrolled in this study. Human leukocyte antigens genotyping were analyzed by polymerase chain reaction sequence specific oligonucleotide technique. RESULTS: The present study revealed significantly high frequency of DQB10101 and DQB10201 alleles among patients in comparison with healthy control, while there was significantly low frequency of each HLA-A3301, B0826, DRB10701, 1101 and HLA-DQB10604 alleles in patients as compared to healthy controls. CONCLUSION: These findings indicate that higher frequency of HLA-DQB10101 and 0201 alleles may be a risk factor for type-1diabetes mellitus, meanwhile low frequency of HLA-A3301, B0826, DRB10701, 1101 and HLA-DQB1-0604 alleles could be a protective factor.
Keywords
Type; HLA; alleles; PCR; SSO

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