Studying the Anti-Adhesion Ability of S-layer Proteins and Filtrate of Lactobacillus spp. Against Some Pathogenic Microorganisms In Vitro

A. Hassan, Raghad

	Studying the Anti-Adhesion Ability of S-layer Proteins and Filtrate of Lactobacillus spp. Against Some Pathogenic Microorganisms In Vitro
Al-Mustansiriyah Journal of Science
Article 1, Volume 22, Issue 5, 2011, Pages 24-40
Author
Raghad A. Hassan
Abstract
This project was conducted to evaluate the activity of S-layer proteins extracted from Lactobacillus in comparison with the activity of concentrated filtrate of Lactobacillus against some pathogenic microorganisms in vitro. Twelve isolates of Lactobacillus spp. obtained from, vinegar, human milk, cow milk, yoghurt and vagina, were used to detect the S-layer protein (Slp) by Sodium Dodecyl Sulfate-Polyacrylamide gel electrophoresis (SDS-PAGE) then extracted it by excised the Slp pand and treated with 6M guanidin hydrochloride (G-HCl) to eluted the protein from gel. The Molecular weights (MW) of Slps were estimated between (37-63 kDa) depending on the Lactobacillus species. The concentrations of Slp were estimated by using a Kit based on the Biuret method. One isolate of each of Lactobacillus acidophilus and Lactobacillus casei, were selected depending on the MW and concentrations of S-layer proteins. Minimum inhibitory concentrations (MICs) of Lactobacillus spp. concentrated filtrates were determined. Results showed 40% and 50% of the concentrated filtrate of both Lactobacilli were the MIC for Pseudomonas aeruginosa, Escherichia coli, respectively, where as MIC for Salmonella typhimurium and Cadida albicans it was 60%, while 60% and 50% of L. acidophilus and L. casei respectively, were MIC against Staphylococcus aureus. At such MIC,s of Lb. spp., adhesion of E. coli and Staph. aureus to the uroepithelial cells was minimized when the average decreases recorded were (5-12) and (4-9) bacteria/cell after they were (50-60) and (29-35) bacteria/cell, respectively. Adhesion of E. coli and Staph. aureus to the uroepithelial cells was also decreased by S-layer proteins with average decreased (3-9) bacteria/cell for both tested bacteria.

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