Erythrocyte Glutathione Transferase Over-Activity and its Correlation with Plasma Homocysteine in Chronic Kidney Diseases

F. Dawood, Kutayba

	Erythrocyte Glutathione Transferase Over-Activity and its Correlation with Plasma Homocysteine in Chronic Kidney Diseases
Al-Mustansiriyah Journal of Science
Article 1, Volume 22, Issue 5, 2011, Pages 93-107
Author
Kutayba F. Dawood
Abstract
Previous study reported increased expression of erythrocyte glutathione transferase (e-GST) in end-stage renal disease patients on maintenance hemodialysis (MHD), and physiological e-GST level in chronic kidney diseases patients under conservative therapy (CKD). Hyperhomocysteinemia is present in more than 90% uremic patients. In present study we re-evaluated the e-GST levels in 72 CKD patients divided into four stages according to the Kidney Disease Outcomes Quality Initiative )K-DOQI( guidelines staging, 62 MHD patients and 80 healthy controls and studied the correlation between e-GST expression and plasma homocysteine (Hcy) levels in MHD patients. The e-GST activity was assayed using a new automated procedure. A new automated spectrophotometric procedure for e-GST activity, validated by intra-day, inter-day and recovery experiments, confirmed an increased e- GST activity in MHD patients (10.2 ± 0.4 U/g Hb) compared with controls (5.8 ± 0.4 U/g Hb). A surprising significant increase of e-GST activity was observed in predialysis patients related to K-DOQI stages (7.4 ± 0.5 U/g Hb, 8 ± 1 U/g Hb, 9.5 ± 0.6 U/g Hb, 12 ± 1 U/g Hb in stages from I to IV, respectively). A direct correlation between plasma Hcy and e-GST expression was found in MHD patients. e-GST proposes as new biomarker for MHD and CKD patients.

Statistics Article View: 114 PDF Download: 70