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Main properties of urease partially purified from seeds of Syrian mesquite (Prosopis farcta) | ||
| Journal of University of Babylon | ||
| Article 1, Volume 22, Issue 3, 2014, Pages 1071-1079 | ||
| Author | ||
| Nazar Abdulameer Hamzah | ||
| Abstract | ||
| Novel sources of urease from leguminosae were selected for activity test, one the them (Prosopis farcta) showed high specific activity (36.52 U mg-1). The active uerase extract then subjected to series of purification steps involved salting out, DEAE, CM ion exchange and size exclusion chromatography. Specific activity were 52.44, 193.27, 230.55, 433.33 U mg-1 respectively. Main features of urease were detected included estimation of molecular weight (95.500 kDa(, optimum pH for activity (8) and stability (7-8), optimum temperature for activity (45oC) and stability (25-30 oC). | ||
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